Biology/Microbiology Laboratory

button-twitter.png button-facebook.png

Biology/Microbiology Laboratory

INTRODUCTION
Based on the Trisakti University policy that focus on the ‘Urban University”, the graduates hoped from  the Program Environmental Technology should be able to solve the Environmental problems, especially the urban environment, as the result of many people activities in the big town as Jakarta.
In operating the education, the Environmental Technology program applies the activities – in class activities, practice in the laboratory, practice drawing/studio, working practice and soon. Especially to the practice implementation, it needs a natural laboratory absolutely to fulfill the students’ need, even in practice, thesis or research facilities to lecturers, and also to serve people.
The biology and microbiology laboratories from the Environmental Technology program have been opened to serve students, lecturers, and public who need those since the even semester 1998/1999. The laboratory activities have been done such as:
  • Introduction to Biology and Microbiology parameters
  • Studying the working technique in the microbiology laboratory
  • Studying the growing of microbe and the factor influenced
  • Studying the roles as bio indicator and biocatalyzator
 AIMS
The aims to do the biology and microbiology laboratory at the Environmental Technology program are for :
1. Serve students to biology and microbiology practice.
2. Serve students to do their thesis
3. Develop science for lecturers around the Environmental Technology Program
4. Supply facilities to serve for public

PRACTICE EVALUATION SYSTEM
The Practice Values consists of Some Factors
1. Preparation, consists of discipline and making journal;
2. Quiz, before practicing time
3. Practice, consists of skills, accuracy, quickness, cleanliness, and discipline in working
4. Report, it is an assignment, observation result or report, consists of presentation technique, calculation, discussion and conclusion.
5. Mid Term Test (written)
6. Final Term Test (written)
The values for each factor are based on the table below:
Table 1. Values for Practice
No. FACTORS FOR SCORING VALUES
1.
2.
3.
4.
5.
6.
Preparation
Quiz
Practice
Report
MID TEST (written)
FINAL TEST (written)
5 %
7,5 %
25 %
12,5 %
20 %
30 %
Total 100 %
Passing Requirements
Practical students will pass/finish the Environmental Biology/Microbiology Practice, if their grades based on the table are not less than C.                                                                                    
For those who haven’t completed the requirements based on the table must repeat depending on their needs.                                                                                                                                        
 
PRACTICE IMPLEMENTATION
Practice
1. Before starting every practice, students have to study every practical procedure (study from the practical direction book) that will be tested in short quiz for 15 minutes.
2. Before practicing, students get the briefing about the analyst procedure to every parameter measured.
3. Students measure and analyze with assistants
4. Take a note to every observation collected in practice
5. Calculate the experiment practice results from the observation results.
6. Make a report that will be submitted to the next practice.

Thesis
1. Make an application to the Head of Laboratory with the permission of the Head of the Program and the Thesis Supervisor.
2. After Being Agreed by the Head of the Laboratory based on the provided room and equipment, students can start doing the thesis in the laboratory.
3. . Students can apply an application to borrow equipment from the laboratory staffs.
4. If students need the laboratory out of the working hours or holiday, they should apply the application to the Faculty General Division to get the agreement from the Dean.
5. Students should return back or pay for the damaged equipment to the staffs after the    research has been finished, clean the equipment and the place for the research provided in the laboratory.

PRACTICE MATERIALS
Once a week the Biology and Microbiology practices at the Environmental Technology Program will be done at certain times, or depend on the need for practice materials such as the observation to microbe, the observation to the behavior of the bio indicator and so on so there is another time out of the practical hours where the students do the observation based on the agreement with the assistants, students and the laboratory staffs who will prepare all students need.

            The practice materials for the environmental biology and microbiology are:
           
Chapter 1. Microbiology Laboratory Technology
Practice 1
  • Laboratory Techniques
  • Microscope Introduction
  • Sterilization of materials and tools
  • Prepare  Medium
  • Microbe Isolate of solid and liquid (water, land, air)
 Preservation of Conserved Morphology
            (bacteria, fungus, protozoa, virus, microalga)              
        
Practice 2

1. Check the result of the  purifying isolate
2. Identify the microbe :
Some coloring techniques :
a. Simple Coloring
b. Gram Coloring
c. Negative Coloring
d. Spore Coloring                                                                                                                                                                                   

Duplicating the subculture to the sloping medium NA

Practice 3
1. Identify the microbes: forming pigment (demonstration)
2. Identify the microbes: forming motions (including to study the medium based on the phase)
3. Identify the microbes: the ecology of the microbes

The microbes interaction with abiotic factors :
  • a. the effect of the antibiotic substance
  • b. the effect of  sunlight
  • c. the effect of metal (the mass of oligodinamic)
Duplicating liquid cultures 100 ml

Practice 4
1. Identify the microbes: the microbes ecology
    The microbes interaction with the abiotic factor:
                  d. the glucose content effect
                  e. the temperature effect to the pigmentation and growth
                  f. the effect of the pH growth
                  g. the effect of the oxygen to the growth
                  h. the chemical effect to the growth
               
2. Duplicate liquid cultures 1000 ml
3. Bio indicator approaching to the community (Plankton & Bentos)

Practice 5
Identification: The microbes interaction with the biotic factors
                  a. The Microbes symbiosis with the taller plants
                       (B.radicicola with R.leguminosorum)
                  b. The  antibiosis around the microbes
                      (B.anthracius dengan pseudomonas aeruginosa)
                  c. The antibiosis around the microbes
                       (E.coli with E.coli)
                  d. Synergism
               
Practice 6
  • 1. Duplicating the microbes : the techniques of the breeding microbes
      1. in aerobic (liquid cultures, solid cultures)                                                                                             
      2. in anaerobic (demonstration)
            2. Checking the water
            a. E.coli as  bio indicator
            b. Isolation E.Coli with mix cultures
The direct and indirect enumeration
Enumerasi langsung dan tidak langsung

Practice 7
Checking Water

            c. The coloring microbes
            d. The result of the checking water demonstration

The Microbes Quantitation
  • a. The direct quantitation with the Neubauer Hemacytometer
  • b. Cup Calculation
 
Practice 8
The making of the growing curves
  • a. with a direction calculation
  • b. spectrophotometer calculation

Praktikum 9
Roles of the microbes in nitrogen cycles on the ground
  • a. Nitrogen Fixation
  • b. Nitrification
  • c. Denitrification
  • d. Ammonification
Practice 10

           BIOESSAY
Penerimaan Mahasiswa Baru Trisakti

icon-twitter.pngRecent Tweets

Updated > Informasi terbaru yang diterima: FEB memberikan kabar yang sama dengan FK, FKG, FTI, FTSP, FH, FTKE, FSRD… https://t.co/hhcsC61wfh
Thursday, 23 May 2019 06:06
Informasi terbaru yang diterima: FK, FKG, FTI, FTSP, FH, FTKE, FSRD, FALTL USAKTI telah diliburkan. Penjelasan lebi… https://t.co/7lbmfuwH6D
Thursday, 23 May 2019 00:12
Info untuk sivitas akademika dan Keluarga Besar Universitas Trisakti: Kamis, 23 Mei 2019 dinyatakan sebagai Hari Li… https://t.co/AcrkPxw5po
Wednesday, 22 May 2019 21:09
#usakti #trisakti (test)
Wednesday, 22 May 2019 20:08
twitter @trisakti_ test
Wednesday, 22 May 2019 20:08
Reposted from @manajemenkampus Pengumuman Universitas Trisakti: RALAT Libur dalam rangka merayakan Idul Fitri 1440… https://t.co/lM7LBKVWA1
Monday, 20 May 2019 15:03
Reposted from @manajemenkampus - Pengumuman Universitas Trisakti: Rabu, 22 Mei 2019 dinyatakan sebagai Hari Libur… https://t.co/bU6TOwwm7a
Monday, 20 May 2019 14:02
Prof. dr. Ali Ghufron Mukti MSc.,Ph.D. (lahir di Blitar, Jatim 17 Mei 1962) adalah Plt. Rektor Universitas Trisakti… https://t.co/8vtTbXvHgo
Friday, 17 May 2019 15:03
Kami turut berbelasungkawa bagi keluarga yang ditinggalkan dan juga kepada komunitas muslim di Selandia Baru.… https://t.co/GbBgF7NUtN
Saturday, 16 Mar 2019 10:10
Reposted from MANAJEMEN KAMPUS UNIVERSITAS TRISAKTI - Diklat Tenaga Keamanan Universitas Trisakti Kampus Nagrak Cia… https://t.co/blKu1tjtta
Thursday, 7 Mar 2019 12:12
Reposted from @alumni_trisakti - Datang Ya, Kumpul Kita semua! REUNI AKBAR ALUMNI UNIVERSITAS TRISAKTI 17 Feb 2019… https://t.co/UOAqC2J9NX
Sunday, 17 Feb 2019 07:07
via @alumni_trisakti : Datang Ya, Kumpul Kita semua! REUNI AKBAR ALUMNI UNIVERSITAS TRISAKTI 17 Feb 2019 Istora Se… https://t.co/vOjzovw758
Saturday, 16 Feb 2019 21:09
Pernyataan Sikap Ikatan Alumni Universitas Trisakti terkait #Pilpres dan #Pileg 2019. sumber: Ikatan Alumni Univer… https://t.co/UtnIP3B1LD
Sunday, 13 Jan 2019 11:11
Pernyataan Sikap Ikatan Alumni Universitas Trisakti terkait #Pilpres dan #Pileg 2019. source: @alumni_trisakti cc:… https://t.co/R1F7GGLoKn
Sunday, 13 Jan 2019 10:10
Selamat Pagi Bapak/Ibu Dosen, Karyawan, dan Mahasiswa/i Universitas Trisakti. Hari ini Kamis, 3 Januari Kampus akti… https://t.co/vdwVSa3wIH
Thursday, 3 Jan 2019 05:05
Innalillahi wa inna ilaihi rojiun. Telah berpulang Bpk Muhammad Lindu bin Hasan (Dosen Teknik Lingkungan, mantan Wa… https://t.co/G19KPiVka8
Saturday, 29 Dec 2018 09:09
Reposted from @manajemenkampus - #tetapkerja #tetapmelayani (persiapan kampus kembali masuk tgl 3 Jan 2019). Teri… https://t.co/FuwDzQB65b
Thursday, 27 Dec 2018 14:02
Universitas Trisakti, kami selalu mencintaimu 💙 #weloveusakti #trisaktipictures #usakti #trisakti #akreditasiAhttps://t.co/ywvhbnTmr9
Tuesday, 25 Dec 2018 06:06
Damai di hati, damai di dunia. Dari mata tersurat makna. Dari hati tersurat kata. Dari pesan tersurat ucapan. Unive… https://t.co/89HZqqwf07
Tuesday, 25 Dec 2018 06:06
Selamat Hari Natal bagi Keluarga Besar Universitas Trisakti yang merayakannya. #natal #natal2018 #christmashttps://t.co/qFSgTVjeGX
Tuesday, 25 Dec 2018 05:05

icon-fb.png Recent Posts

Info untuk sivitas akademika dan Keluarga Besar Universitas Trisakti: Kamis, 23 Mei 2019 dinyatakan sebagai Hari Libur Fakultatif.

#usakti #trisakti #universitastrisakti #trisaktiuniversity

Updated > Informasi terbaru yang diterima: FEB memberikan kabar yang sama dengan FK, FKG, FTI, FTSP, FH, FTKE, FSRD, dan FALTL USAKTI yg diliburkan pada tgl 23 Mei 2019. Penjelasan lebih detail silahkan konfirmasi kpd #humas_usakti atau koordinator fakultas masing-masing.
Wednesday, 22 May 2019 21:09
Reposted from MANAJEMEN KAMPUS UNIVERSITAS TRISAKTI Pengumuman Universitas Trisakti: RALAT Libur dalam rangka merayakan Idul Fitri 1440 H. #usakti #trisakti #universitastrisakti #trisaktiuniversity
Monday, 20 May 2019 14:02
Reposted from MANAJEMEN KAMPUS UNIVERSITAS TRISAKTI - Pengumuman Universitas Trisakti: Rabu, 22 Mei 2019 dinyatakan sebagai Hari Libur Fakultatif (mengingat kondisi dan situasi menjelang diumumkannya hasil #pilpres2019). Khusus untuk Rektorat Rabu, 22 Mei 2019 dinyatakan sebagai Hari Libur. #usakti #trisakti #universitastrisakti #trisaktiuniversity
Monday, 20 May 2019 14:02
Prof. dr. Ali Ghufron Mukti M.Sc.,Ph.D. (lahir di Blitar, Jawa Timur, 17 Mei 1962) adalah Plt. Rektor Universitas Trisakti / Direktur Jenderal Sumber Daya Ilmu Pengetahuan Teknologi dan Pendidikan Tinggi (SDID), Kementerian Riset,Teknologi dan Pendidikan Tinggi (Kemenristekdikti) Republik Indonesia. Beliau pernah menjabat sebagai Wakil Menteri Kesehatan Republik Indonesia pada Kabinet Indonesia Bersatu II dan juga Pejabat sementara Menteri Kesehatan Republik Indonesia.

#rektorusakti #alighufronmukti #usakti #trisakti #universitastrisakti #trisaktiuniversity

sumber foto: Sumber Daya Kementerian Riset, Teknologi dan Pendidikan Tinggi
caption: Wikipedia
Friday, 17 May 2019 13:01
Kami turut berbelasungkawa bagi keluarga yang ditinggalkan dan juga kepada komunitas muslim di Selandia Baru.

#usaktiberdoa
#prayformuslimnewzealand
#prayformuslimselandiabaru
#prayfornewzealand
#prayfornz
#usakti #trisakti
#universitastrisakti
#trisaktiuniversity
Saturday, 16 March 2019 10:10
Kami turut berbelasungkawa bagi keluarga yang ditinggalkan dan juga kepada komunitas muslim di Selandia Baru.

#usaktiberdoa
#prayformuslimnewzealand
#prayformuslimselandiabaru
#prayfornewzealand
#prayfornz
#usakti #trisakti
#universitastrisakti
#trisaktiuniversity
Saturday, 16 March 2019 10:10
Reposted from MANAJEMEN KAMPUS UNIVERSITAS TRISAKTI - Diklat Tenaga Keamanan Universitas Trisakti Kampus Nagrak Ciangsana (7-9 Maret 2019)

" Membangun Korsa dan Jati Diri Tenaga Keamanan Universitas Trisakti yang Tangguh, Disiplin dan Bertanggungjawab "

Pendidikan dan Latihan ini dibuka oleh Direktur Kampus, Dr. Ir. Hardi Utomo, MS, IAI.

https://www.instagram.com/p/BusaU8jlvM1/?utm_source=ig_share_sheet&igshid=1cu9gkjq7u6vu
Thursday, 7 March 2019 12:12
Reposted from Ikatan Alumni Universitas Trisakti (IKAUSAKTI) - Datang Ya, Kumpul Kita semua! REUNI AKBAR ALUMNI UNIVERSITAS TRISAKTI

17 Feb 2019
Istora Senayan

Let’s bring our memories back!
Trisakti Connect Fest
#TCF2019
#trisakticonnect
#trisakticonnectfestival
#trisakticonnectfestival2019
#reuniakbar
#reuniakbar_usakti
#Ikausakti #ikatanalumniuniversitastrisakti #alumniusakti #alumnitrisakti #usakti #trisakti #universitastrisakti #trisaktiuniversity
Sunday, 17 February 2019 07:07
Pernyataan Sikap Ikatan Alumni Universitas Trisakti terkait #Pilpres dan #Pileg 2019.

sumber: Ikatan Alumni Universitas Trisakti (IKAUSAKTI)
cc: KPU Republik Indonesia Bawaslu RI Dewan Kehormatan Penyelenggara Pemilu Republik Indonesia

#pilpres2019 #pileg2019
#Ikausakti #alumniusakti #alumnitrisakti #usakti #trisakti #universitastrisakti #trisaktiuniversity
Sunday, 13 January 2019 11:11
Selamat Pagi Bapak/Ibu Dosen, Karyawan, dan Mahasiswa/i Universitas Trisakti. Hari ini Kamis, 3 Januari Kampus aktif kembali usai liburan. Semangat di Tahun Baru 2019.
Thursday, 3 January 2019 05:05
Reposted from MANAJEMEN KAMPUS UNIVERSITAS TRISAKTI - #tetapkerja #tetapmelayani (persiapan kampus kembali masuk tanggal 3 Januari 2019).

Terima Kasih atas kerjasama dan koordinasi yg baik dalam rangka mewujudkan keamanan dan ketertiban serta penjagaan dan pengamanan sarana dan prasarana kampus.

#usakti #trisakti
#trisaktiuniversity
#universitastrisakti
Thursday, 27 December 2018 13:01
Universitas Trisakti, kami selalu mencintaimu 💙

#weloveusakti
Welcome to Trisakti University
#trisaktipictures
#usakti #trisakti
#akreditasiA
#trisaktiuniversity
#universitastrisakti
Tuesday, 25 December 2018 06:06
Damai di hati, damai di dunia. Dari mata tersurat makna. Dari hati tersurat kata. Dari pesan tersurat ucapan.

Universitas Trisakti mengucapkan: “Rahajeng Nyanggra rahina Galungan lan Kuningan" Selamat Hari Raya Galungan dan Kuningan (26 Desember 2018 dan 5 Januari 2019)

#galungan #kuningan #galungankuningan #galunganlankuningan #usakti #trisakti #trisaktiuniversity #universitastrisakti
Tuesday, 25 December 2018 06:06
Selamat Hari Natal bagi Keluarga Besar Universitas Trisakti yang merayakannya.

#natal #natal2018 #christmas #christmas2018
#usakti #trisakti #universitastrisakti #trisaktiuniversity
Tuesday, 25 December 2018 05:05
Mari kita berdoa untuk
saudara-saudara kita. Doa untuk #Indonesia
Semoga diberikan perlindungan dan keselamatan.

#UsaktiBerdoa #prayforindonesia #prayforanyer #prayforbanten #prayforlampung

Reposted from Badan Nasional Penanggulangan Bencana - #SahabatTangguh, Benar, Tsunami Menerjang Pantai di Selat Sunda

BMKG telah menyampaikan secara resmi bahwa tsunami telah terjadi dan menerjang beberapa wilayah pantai di Selat Sunda, diantaranya di pantai di Kabupaten Pandeglang, Serang, dan Lampung Selatan. Tsunami terjadi pada 22/12/2018 sekitar pukul 21.27 WIB.
Sunday, 23 December 2018 07:07

Guru Besar

Prof. Dr. Wahyudi Wisaksono Faculty of Landscape Architecture and Environmental Technology

Nama Lengkap Prof. Dr. Wahyudi Wisaksono : Pangkat / Golongan Guru Besar IV/e : Fakultas / Jurusan / Bagian Arsitektur…

Hubungi Kami


Jalan Kyai Tapa No. 1 Grogol
Jakarta Barat, Indonesia
Phone: (62-21) 566 3232 Ext 8762, 8765
Fax: (62-21) 560 2575
Email : tekniklingkungan@trisakti.ac.id
Email: humas@trisakti.ac.id

Alumni